How much bacteriostatic water should be added to a 40mg MOTS-c vial?

There is no single correct volume — the amount of water decides the concentration, and the concentration decides how legible the result is on the barrel of your syringe. For a 40mg vial: 2mL gives 20mg/mL, and 3mL gives 13.33mg/mL. More water means a lower concentration and a longer, easier-to-read draw; less water means the vial lasts across more draws. Bacteriostatic water contains a preservative, so a vial reconstituted with it can be drawn from repeatedly.

What concentration is 40mg of MOTS-c in 3mL of bacteriostatic water?

40mg dissolved in 3mL gives a concentration of 13.33mg per mL. A U-100 insulin syringe holds 100 units per mL, so each unit on the barrel contains 133.33mcg, and the reconstituted vial holds 300 units of solution in total.

How many units on an insulin syringe is 1mL of reconstituted MOTS-c?

On a standard U-100 insulin syringe, 1mL is 100 units — that is what U-100 means, and it is true regardless of what is dissolved in the liquid. A 0.5mL syringe is marked to 50 units and a 0.3mL syringe to 30 units, at the same 100-units-per-mL scale. What changes with the compound is how much material each unit carries, which is the concentration figure above.

MOTS-c Reconstitution Calculator

Enter how much bacteriostatic water you added to your 40mg MOTS-c vial and the amount you want per draw. The calculator reports the concentration, where the draw lands on an insulin syringe, and how many draws the vial holds.

What is the total volume of your syringe?

Printed on the barrel. Insulin syringes are marked in units, at 100 units to the millilitre.

How many milligrams are in the vial?

The figure on the label, before any water is added.

How much bacteriostatic water are you adding?

We reconstitute at 3 mL as standard. More water does not change the total peptide, only how far it is spread.

How much peptide do you want in each draw?

For reference, 500 mcg = 0.5 mg, 1,000 mcg = 1 mg.

To draw 250 mcg, pull the plunger to 1.9 units on your 1.0 mL syringe.

Concentration: 13.33 mg/mL
Per unit: 133.3 mcg
Draw volume: 0.019 mL
Draws per vial: 160

For research purposes only. Not for human consumption. All products are sold strictly as in-vitro laboratory reagents to qualified researchers, and are not drugs, foods, cosmetics or medical devices. They are not intended to diagnose, treat, cure or prevent any disease, and must not be administered to humans or animals. You must be 21 or older to purchase.

Reconstituting a vial

Lyophilised powder has to be dissolved before any of the arithmetic above applies. These are general laboratory handling notes for research material.

Work on a wiped-down surface with nothing on it but what the procedure needs. Sterility is the whole game here: a solution that picks up contamination on the bench stays contaminated for the entire life of the vial, and nothing later in the process removes it.

Have the lyophilised vial, bacteriostatic water, a sterile syringe, alcohol swabs and gloves laid out before you start, so that no step is done one-handed while reaching for something.

Take the powder and the solvent out of cold storage and let them come up to room temperature before combining them. Cold solvent dissolves lyophilised material slowly and unevenly, which is what produces the stubborn clumps that get mistaken for a bad vial.

Swab the rubber stopper on both vials with alcohol and let it dry rather than wiping it off.

Draw the volume you entered above, then hold the vial at an angle and let the water run down the inside wall rather than jetting it directly onto the powder. Peptides are fragile in solution, and a hard stream foams them.

Let it dissolve on its own. Swirl gently if it needs help. Do not shake — foam on the surface is denatured material, and it is not coming back.

Unreconstituted powder is the stable form and should stay cold and dark until you need it. Once it is in solution the clock starts: refrigerated, most peptides hold for a few weeks; frozen, for a few months.

Freeze in single-use aliquots rather than repeatedly thawing one vial. Each freeze-thaw cycle costs potency, and the loss does not announce itself — the solution looks identical either way.

Looking for a particular compound? Browse the catalogue, or ask us what documentation exists for the lot in stock.

About MOTS-c

MOTS-c is a 16-amino-acid peptide encoded in the mitochondrial genome rather than the nuclear one, which makes it one of a small class of mitochondrial-derived peptides. It is studied in research on metabolic homeostasis and AMPK signalling.

Reconstituting a 40mg vial

40mg sits in the awkward middle of this catalog: too concentrated to work like a 10mg vial, not obviously large enough to prompt anyone to check. 3mL gives 13.33mg/mL and 133.3mcg per unit, so draws that would be 20 units from a 10mg vial become 5 units here. Anything the calculator reports in low single-digit units is worth re-running with more water, because the barrel simply is not printed finely enough to read it.

40mg MOTS-c concentration chart

What a 40mg vial becomes at four common reconstitution volumes, on a U-100 insulin syringe.

Bacteriostatic water	Concentration	Per unit on the barrel	Total units in the vial
1mL	40mg/mL	400mcg	100 units
2mL	20mg/mL	200mcg	200 units
3mL	13.33mg/mL	133.33mcg	300 units
5mL	8mg/mL	80mcg	500 units

A U-100 insulin syringe is marked at 100 units to the millilitre. Concentration is the vial strength divided by the volume of water added; nothing on this chart is a recommendation about how much of that solution to use.

MOTS-C from Ascend Vials

Sourced against a 99%+ identity purity specification, shipped anywhere in the continental United States for a flat $4.99 and arriving in 2–4 days. For in-vitro laboratory research only.

View MOTS-C — from $39.99 Bacteriostatic water

MOTS-c reconstitution questions

How much bacteriostatic water should be added to a 40mg MOTS-c vial?: There is no single correct volume — the amount of water decides the concentration, and the concentration decides how legible the result is on the barrel of your syringe. For a 40mg vial: 2mL gives 20mg/mL, and 3mL gives 13.33mg/mL. More water means a lower concentration and a longer, easier-to-read draw; less water means the vial lasts across more draws. Bacteriostatic water contains a preservative, so a vial reconstituted with it can be drawn from repeatedly.
What concentration is 40mg of MOTS-c in 3mL of bacteriostatic water?: 40mg dissolved in 3mL gives a concentration of 13.33mg per mL. A U-100 insulin syringe holds 100 units per mL, so each unit on the barrel contains 133.33mcg, and the reconstituted vial holds 300 units of solution in total.
How many units on an insulin syringe is 1mL of reconstituted MOTS-c?: On a standard U-100 insulin syringe, 1mL is 100 units — that is what U-100 means, and it is true regardless of what is dissolved in the liquid. A 0.5mL syringe is marked to 50 units and a 0.3mL syringe to 30 units, at the same 100-units-per-mL scale. What changes with the compound is how much material each unit carries, which is the concentration figure above.